The airline, which will be merged with Tata-owned Air India, faced similar disruptions last month.
Vistara said on Monday it was forced to cut a number of flights as it was dealing with a shortage of pilots and crew.
“We have had a significant number of flight cancellations and delays in the past few days due to various reasons including crew unavailability,” a company spokesperson said in a statement.
Vistara, owned by Tata Group and Singapore Airlines, is using larger aircraft, including the wide-body Boeing 787 Dreamliner on certain domestic routes “to accommodate more passengers, wherever possible,” the spokesperson said.
The airline, which will be merged with Tata-owned Air India, faced similar disruptions last month.