It is the second time the convicted murderer has escaped, the first time in 2013. President Daniel Noboa said his government will focus on tackling crime inside the country’s prisons.
A state of emergency has been declared in Ecuador after one of the country’s most-notorious drug gang bosses escaped from prison.
Adolfo Macias – also known as Fito – was first reported missing from his cell on Sunday.
Ecuador’s prosecutors then filed charges against two prison guards as part of their investigation into the alleged escape.
The leader of the Los Choneros gang was serving a 34-year sentence in La Regional prison for drug trafficking and murder.
His reported escape occurred on the same day he was scheduled to be transferred to a maximum security facility in the city of Guayaquil.
President Daniel Noboa announced the state of emergency, which will allow the authorities to suspend citizens’ rights and send the military into prisons.
The decree is in effect for 60 days, with Mr Noboa saying he will confront crime inside prisons and will not stop until he “brings back peace to all Ecuadorians”.
In 2013, Macias fled a maximum security prison but was recaptured a few weeks later. Police are investigating whether his most recent escape mirrors his one from a decade ago.
Los Choneros is one of the gangs authorities consider responsible for a spike in violence that reached new highs last year with the assassination of the presidential candidate Fernando Villavicencio.